Ethoxy or methoxyl silane functionalized polyethylene glycol, silane PEG (PEG-Si) is a surface reactive PEG derivative that can be used to modify glass, silica and other surfaces via the reaction between hydroxyl group and ethoxyl/methoxyl silane. Pegylation can greatly suppress the non-specific binding of charged molecules to the modified surfaces. And they have wide applications for medica device, biomems or biocompatible material development.
物理特性:
灰白色/白色固體或粘性液體取決于分子量;
溶于常規(guī)水溶液和大多數有機溶劑;
Off-white/white solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Generally, silane PEG is first dissolved in a mixture of ethanol/water solution, then allow the solution to contact with oxidized glass or silica surface for 30 min to 2 hours. Under this condition, silane PEG should bind to the material covalently.
所需材料:
聚乙二醇化溶液:乙醇/水(w/w,95%/5%);
硅烷PEG儲備液,10~50毫克/毫升聚乙二醇化溶液,新鮮制備。
洗滌液:蒸餾水。
Pegylation solution: Ethanol/water (w/w, 95%/5%);
Silane PEG stock solution, 10~50 mg/mL in pegylation solution, prepare in fresh.
1. Dissolve targeted materials in Pegylation buffer.
2. Add silane PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of silane PEG needed for optimal conjugation;
3. Allow mixture agitates at room temperature for 30 min at room temperature or 2 hours at 4 -20 0C.
4. Wash out unreacted materials either by distilled water.